Most LTPs are relatively stable to heating and proteolysis (Asero et al. 2000 [10]) whilst the stability of hazelnut ns-LTP has not been characterised. Wigotzki et al. (2000) [1119] found that a hazelnut allergen <14 kDa was very stable to heating. Pastorello et al. (2002) [671] have shown that extracts from both native and roasted hazelnuts inhibit IgE binding from Cor a 8 sensitised patients to native hazelnut by EAST inhibition assay.
Nature of main cross-reacting proteins:
Not known. However, cross-reactivity with other ns-LTPs is likely.
Allergen properties & biological function:
Non-specific lipid transfer protein, nsLTP. May be involved in wax or cutin deposition. LTPs have four alpha-helices around a central expandable cavity which binds lipid. The helices are linked by four disulphide bridges. nsLTPs have also been reported to act as plant defense proteins against bacterial and fungal infections and form the PR14 family of pathogenesis related proteins.
Allergen purification:
Schocker et al. (2004) [951] partially purified Cor a 8 by ion exchange chromatography using DEAE Sepharose Fast Flow Resin with an elution buffer of 20 mmol/L Tris and 0.5 mol/L NaCl, pH 8.0.
Schocker et al. (2004) [951] also expressed complementary DNA encoding the mature protein Cor a 8 in E coli BL21(DE3) RIL cells at 30°C for 2 hours. Purification was performed under native conditions by means of metal affinity chromatography. Approximately 1.5 mg of recombinant protein was purified from 1 L of bacterial culture.
Other biochemical information:
References (6)
Asero R, Mistrello G, Roncarolo D, Amato S, Caldironi G, Barocci F, van Ree R.
Immunological cross-reactivity between lipid transfer proteins from botanically unrelated plant-derived foods: a clinical study.
Allergy 57(10):900-906 2002
PUBMED ID:
12269935
Asero R, Mistrello G, Roncarolo D, de Vries SC, Gautier MF, Ciurana CL, Verbeek E, Mohammadi T, Knul-Brettlova V, Akkerdaas JH, Bulder I, Aalberse RC, van Ree R.
Lipid transfer protein: a pan-allergen in plant-derived foods that is highly resistant to pepsin digestion.
Int Arch Allergy Immunol. 122:20-32. 2000
PUBMED ID:
10859466
Schocker F, Luttkopf D, Scheurer S, Petersen A, Cistero-Bahima A, Enrique E, San Miguel-Moncin M, Akkerdaas J, van Ree R, Vieths S, Becker WM.
Recombinant lipid transfer protein Cor a 8 from hazelnut: a new tool for in vitro diagnosis of potentially severe hazelnut allergy.
J Allergy Clin Immunol. 113(1):141-147. 2004
PUBMED ID:
14713920
Wigotzki M, Steinhart H, Paschke A.
Influence of varieties, storage and heat treatment on IgE-binding proteins in hazelnuts (Corylus avellana).
Food Agric Immunol. 12: 217-229.
2000
PUBMED ID:
unknown
[1119]
This record was last modified on 18-Oct-2006
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