Biochemical Data: Egg (hen's egg) and Apovitellenin VI

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BIOCHEMICAL INFORMATION for Egg (hen's egg)

Allergen Name:

Apovitellenin VI

Alternatve Allergen Names:

Apoprotein B

Allergen Designation:

Minor

Protein Family:

Not known. The N-terminus of apoB contains a vitellogenin_N domain http://www.sanger.ac.uk//cgi-bin/Pfam/getacc?PF01347.

Sequence Known?:

A fragment of apoB has been deposited. The chicken genome contains a related 4204 amino acid predicted sequence which probably corresponds to complete apoB.

Allergen accession No.s:

http://us.expasy.org/uniprot/P11682 http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=XP_419979.1

3D Structure Accession No.:

N/A

Calculated Masses:

N/A

Experimental Masses:

170 kDa

Oligomeric Masses:

Not known

Allergen epitopes:

Not known

Allergen stability:
Process, chemical, enzymatic

Not known

Nature of main cross-reacting proteins:

Not known

Allergen properties & biological function:

ApoB derived proteins form the majority of egg yolk protein. Lipid binding is a major role.

Allergen purification:

Burley and Sleight (1983) [1524] describe a purification of apovitellenin VI using gel filtration on Separose 6B in 6M urea at pH 3.3 and hydrophobic interaction chromatography.

Other biochemical information:

The name apovitellenin VI is seldom used. Burley and Sleight (1983) [1524] isolated this protein and Evans and Burley (1987) [1521] showed that it was the largest fragment produced by proteolysis of apoprotein B as egg yolk is formed.

References (5)

Anet J, Back JF, Baker RS, Barnett D, Burley RW, Howden ME.
Allergens in the white and yolk of hen's egg. A study of IgE binding by egg proteins. Int Arch Allergy Appl Immunol. 77(3):364-371. 1985
PUBMED ID: 4008088

[1522]

Burley RW, Sleigh RW.
Hydrophobic chromatography of proteins in urea solutions. The separation of apoproteins from a lipoprotein of avian egg yolk. Biochem J. 209(1):143-150. 1983
PUBMED ID: 6847608

[1524]

Evans AJ, Burley RW.
Proteolysis of apoprotein B during the transfer of very low density lipoprotein from hens' blood to egg yolk. J Biol Chem. 262(2):501-504. 1987
PUBMED ID: 3542986

[1521]

Walsh BJ, Barnett D, Burley RW, Elliott C, Hill DJ, Howden ME.
New allergens from hen's egg white and egg yolk. In vitro study of ovomucin, apovitellenin I and VI, and phosvitin. Int Arch Allergy Appl Immunol. 87(1):81-86. 1988
PUBMED ID: 3170012

[1215]

Walsh BJ, Elliott C, Baker RS, Barnett D, Burley RW, Hill DJ, Howden ME.
Allergenic cross-reactivity of egg-white and egg-yolk proteins. An in vitro study. Int Arch Allergy Appl Immunol. 84(3):228-232. 1987
PUBMED ID: 3654006

[1523]

This record was last modified on 18-Oct-2006
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