Biochemical Data: Walnut and Jug r 2

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BIOCHEMICAL INFORMATION for Walnut

Allergen Name:

Jug r 2

Alternatve Allergen Names:

7S globulin; vicilin-like protein

Allergen Designation:

Major

Protein Family:

PFAM PF00190; Cupin; 7S vicilin with an additional N-terminal region PFAM PF04702; Vicilin_N, which is removed on maturation.

Sequence Known?:

Yes

Allergen accession No.s:

http://ca.expasy.org/cgi-bin/niceprot.pl?Q9SEW4

3D Structure Accession No.:

Not determined

Calculated Masses:

48.3 kDa (69990 kDa precursor)

Experimental Masses:

44-47 kDa

Oligomeric Masses:

144.9 kDa trimer

Allergen epitopes:

Not known

Allergen stability:
Process, chemical, enzymatic

Not known

Nature of main cross-reacting proteins:

Not known

Allergen properties & biological function:

7S seed storage globulin

Allergen purification:

A recombinant protein was expressed as a fusion protein with a glutathione-S-transferase (GST) domain. This was then purified on a column of immobilized glutathione (Bulk GST purification module, Pharmacia Biotech).

Other biochemical information:

9/15 sera reacted to recombinant Jug r 2 (Teuber et al. 1999 [207]) inhibiting IgE binding to polypeptides at 44-47 kDa. N-terminal sequencing of the 44 kDa allergen band gave EEEQQRHNPYYFH, which suggests that the Jug r 2 precursor is cleaved after residue 172 to give a 48.3 kDa mature vicilin.

References (1)

Teuber SS, Jarvis KC, Dandekar AM, Peterson WR, Ansari A
Identification and cloning of a complimentary DNA encoding a vicilin-like proprotein, Jug r 2, from English walnut kernel (Juglans regia, a major food allergen. J Allergy Clin Immunol 104:1311-1320. 1999
PUBMED ID: 10589017

[207]

This record was last modified on 18-Oct-2006
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