Biochemical Data: Mustard and Bra j 1

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BIOCHEMICAL INFORMATION for Mustard

Allergen Name:

Bra j 1

Alternatve Allergen Names:

2S Albumins

Allergen Designation:

Major

Protein Family:

Pfam PF00234, 2S seed storage proteins

Sequence Known?:

Yes

Allergen accession No.s:

http://ca.expasy.org/cgi-bin/niceprot.pl?P80207

3D Structure Accession No.:

Not determined

Calculated Masses:

14644 Da for both chains.

Experimental Masses:

16.4 kDa (single chain) 4.3 kDa and 10.0 kDa (reduced) (Gonzalez de la Peña 1991 [646])

Oligomeric Masses:

Not known

Allergen epitopes:

IgE epitope: 55-QLQHEISRIYQTAT-68 in the sequence of Bra j 1E is likely to bind IgE as it is similar to the epitope of Sin a 1, QGPHVISRIYQTAT, and binds the same monoclonal IgG antibody (Monsalve et al., 1993 [121]).

Allergen stability:
Process, chemical, enzymatic

Bra j 1, is a 2S albumin, constituted by two polypeptide chains of 4.3 and 10 kDa. Being a 2S albumin, it probably shares stability properties of other proteins of this type on heating (see data for Sin a 1, the yellow mustard allergen). It is resisant to proteolytic digestion (Astwood et al. 1996 [1160]).

Nature of main cross-reacting proteins:

See data for Sin a 1

Allergen properties & biological function:

See data for Sin a 1. The N-terminus forms pyroglutamate.

Allergen purification:

In summary delipidation of crushed seeds, aqueous extraction, gel filtration (Sephadex G50) allows the separation of the 2S fraction. Ion exchange chromatography (SP-Sephadex C25) separates five isoallergenic fractions, named Bra j 1A to Bra j 1E (Gonzalez de la Peña et al, 1991 [646]).

Other biochemical information:

References (7)

Astwood JD, Leach JN, Fuchs RL.
Stability of food allergens to digestion in vitro. Nat Biotechnol. 14(10):1269-1273. 1996
PUBMED ID: 9631091

[1160]

Gonzalez de la Peña MA, Menendez-Arias L, Monsalve RI, & Rodriguez R
Isolation and characterization of a major allergen from oriental mustard seeds, BrajI Int Arch Allergy Appl Immunol 96: 263-270. 1991
PUBMED ID: 1804799

[646]

Gonzalez De La Pena MA, Monsalve RI, Batanero E, Villalba M, Rodriguez R.
Expression in Escherichia coli of Sin a 1, the major allergen from mustard. Eur J Biochem 237: 827-832. 1996
PUBMED ID: 8647131

[61]

Menendez-Arias L, Dominguez J, Moneo I, Rodriguez R.
Epitope mapping of the major allergen from yellow mustard seeds, Sin a I. Mol. Immunol. 27: 143-150. 1990
PUBMED ID: 1690853

[109]

Menendez-Arias L, Moneo I, Dominguez J, Rodriguez R.
Primary structure of the major allergen of yellow mustard (Sinapis alba L.) seeds, Sin a I. Eur. J. Biochem. 177:159-166. 1988
PUBMED ID: 3181153

[111]

Monsalve RI, Villalba M, Lopez-Otin C, Rodriguez R.
Characterization of a new oriental-mustard (Brassica juncea) allergen, Bra j IE: detection of an allergenic epitope. Biochem. J. 293: 625-632. 1993
PUBMED ID: 7688955

[121]

Onaderra M, Monsalve RI, Mancheno JM, Villalba M, Martinez del Pozo A, Gavilanes JG, Rodriguez R.
Food mustard allergen interaction with phospholipid vesicles. Eur. J. Biochem. 225: 609-615. 1994
PUBMED ID: 7957175

[134]

This record was last modified on 18-Oct-2006
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