There are many reports of one or two cases of allergy to either grapes or wine, the single article of Pastorello et al. (2003) [707] reporting 14 severe cases.

• Exercise-induced anaphylaxis after eating grapes (Dohi et al 1991 [318]).
• Palmoplantar pruritus, urticaria, dyspnea, dysphagia, gastric pain, sweating, and tachycardia (Bircher et al. 1999) [869]
• Oral allergy syndrome to grapes and wine (Giannoccaro et al 1998 [341]).
• Two cases of oral allergy syndrome to grapes: one of them with an episode of anaphylaxis and the other with exercise-induced anaphylaxis after eating grapes (Guinnepain et al. 1998 [348]).
• A woman with two episodes of urticaria, angioedema and diyspnea and one of anaphylaxis (Vaswani et al 1999 [551]).
• Exercise-induced anaphylaxis with wine (Garcia-Robaina et al 2001 [340]).
• Facial flushing, edema of lips, and dyspnea after drinking white or red wine but could eat fresh grapes without a problem. However, exercise-induced anaphylaxis after eating white grapes and then exercise. After exercise, urticaria, facial/pharyngeal edema, abdominal pain, and dyspnea appeared and rapidly worsened (Senna et al 2001 [706])
• Oral allergy syndrome and lip angioedema in a 5-year-old child after eating grapes (Rodriguez et al 2001 [705])
• Oral allergy syndrome in a 4 year old boy after the consumption of grapes (Petrus & Malandain 2002 [709])
• Anaphylaxis after eating white grapes (Anton et al 1997 [961]; Caiaffa et al 2003 [708])
• Pastorello et al. (2003) [707] reported 14 adult patients with severe symptoms, 11 to grape and 3 to wine. Two showed OAS and others angioedema, laryngeal edema, urticaria, gastrointestinal symptoms, hypotension, asthma and anaphylactic shock. One case of anaphylactic shock after exercise.

• Rodriguez et al (2004) [960] reported 14 patients with OAS (6/14), anaphylaxis (4/14), angioedema (2/14), urticaria (1/14) and urticaria-angioedema (1/14)

Commercial grape extract and fresh grapes (Anton et al. 1997) [961].

Red grapes (Guinnepain et al. 1998) [348].

Fresh red and white grapes and dried grapes (Giannoccaro et al. 1998) [341].

Grape extract (Vaswani et al. 1999) [551]

Raisins and grape juice and with different species of fresh grapes (Bircher et al. 1999) [869].

Pulp and peel of fresh white grape (Moscatel variety) and pulp and peel of blue grape (Rodriguez et al. 2001) [705].

White and red grape juice (Senna et al. 2001) [706].

Fresh grape (Garcia-Robaina et al. 2001 [340], Pastorello et al. 2003) [707]

Commercial grape extract, white fresh grapes and white grape juice (Caiaffa et al. 2003) [708].

Commercial grape extract and pulp or peel from fresh grape (Rodriguez et al. 2004) [960].

Prick to prick test (Guinnepain et al. 1998 [348], Giannoccaro et al. 1998 [341], Garcia-Robaina et al. 2001) [340].

The skin test was positive with a 10 mm wheal and 14 mm erythema above the saline control (Vaswani et al. 1999) [551].

Prick-to-prick. The test was defined as positive with a wheal diameter of at least 3 mm (Bircher et al. 1999) [869].

Prick-plus-prick technique. A positive skin reaction was defined as the presence of a wheal larger than that elicited with the histamine phosphate control as read after 20 minutes (Pastorello et al. 2003) [707]

Prick test and prick-by-prick test. The tests were positive when a wheal diameter of at least 3 mm was observed (Caiaffa et al. 2003) [708].

3 patients who suffered anaphylactic reaction after eating fresh grapes (Anton et al., 1997) [961].

Two patients. One of them experienced anaphylactic shock and the second patient developed exercise-induced anaphylaxis (Guinnepain et al. 1998) [348].

A 24-year-old man with no family history of allergy suffered from oral allergy syndrome (OAS) after eating fresh grapes and drinking either white or red wine (Giannoccaro et al. 1998) [341].

A 28-year-old woman (Vaswani et al. 1999) [551].

A 44-year-old man (Bircher et al. 1999) [869].

A 5-year-old female (Rodriguez et al. 2001) [705].

A 33-year-old woman showing exercise-induced anaphylaxis (Senna et al. 2001) [706].

A 19-year-old man (Garcia-Robaina et al. 2001) [340].

Fourteen patients, 10 women and 4 men (mean age, 30.5 years; age range 23-47 years), were selected on the basis of a documented history of severe allergic reactions to grape (11 patients) or wine (3 patients) (Pastorello et al. 2003) [707]

A 28-year-old woman with allergic systemic reaction after eating white grapes (Caiaffa et al. 2003) [708].

14 children with allergic reactions after eating either fresh grapes or grape juice (Rodriguez et al. 2004) [960].

Positive for 3 patients (Anton et al. 1997) [961]

Positive for 2 patients (Guinnepain et al. 1998) [348].

The test was highly positive with the three fruits (Giannoccaro et al. 1998) [341].

Positive (Vaswani et al. 1999) [551].

Test was positive for grapes, whereas other grapes were skin-test negative (Bircher et al. 1999) [869].

The test was positive (Rodriguez et al. 2001) [705].

Positive (Senna et al. 2001) [706].

Positive (Garcia-Robaina et al. 2001) [340].

SPTs with fresh grape provided positive results to all the patients (Pastorello et al. 2003) [707].

The skin prick test performed with commercial extract was negative but the prick-by-prick performed with white grapes and juice was positive (Caiaffa et al. 2003) [708].

The skin prick test performed with commercial extract was positive for one patient, but the tests performed with white pulp and peel grape were positive for 4 patients (Rodriguez et al. 2004) [960]

Anton et al. 1997 [961] used commercial discs (Immuno system CAP) and nitrocellulose discs coupled with their own extracts (RAST)

CAP-RAST (Guinnepain et al. 1998) [348], Vaswani et al. 1999 [551], Senna et al. 2001 [706], Caiaffa et al. 2003 [708]

RAST (Giannoccaro et al. 1998) [341].

CAP (Bircher et al. 1999 [869], Rodriguez et al. 2001 [705], Pastorello et al. 2003 [707]).

3 patients who suffered anaphylactic reaction after eating fresh grapes (Anton et al., 1997) [961].

Two patients. One of them experienced anaphylactic shock and the second patient developed exercise-induced anaphylaxis (Guinnepain et al. 1998) [348].

A 24-year-old man with no family history of allergy suffered from oral allergy syndrome (OAS) after eating fresh grapes and drinking either white or red wine (Giannoccaro et al. 1998) [341].

A 28-year-old woman (Vaswani et al. 1999) [551].

A 44-year-old man (Bircher et al. 1999) [869]

A 5-year-old female (Rodriguez et al. 2001) [705].

A 33-year-old woman showing exercise-induced anaphylaxis (Senna et al. 2001) [706].

A 19-year-old man (Garcia-Robaina et al. 2001) [340].

A 28-year-olg woman with allergic systemic reaction after eating white grapes (Caiaffa et al. 2003) [708].

Positive for all patients (Anton et al. 1997) [961].

Positive in both patients (Guinnepain et al. 1998) [348].

Serum gave a positive class 2 RAST (Giannoccaro et al. 1998) [341].

Grape-specific serum IgE level was weakly positive (class 1) by RAST and negative by CAP (Vaswani et al. 1999) [551].

No grape-specific IgE could be demonstrated in the patient’s serum (Bircher et al. 1999) [869].

Specific IgE to grape was 1.1 KU/l (Rodriguez et al. 2001) [705].

No grape-specific IgE could be demonstrated in the patient’s serum (Senna et al. 2001) [706].

Specific IgE to grape was 4.13 KU/ml (Garcia-Robaina et al. 2001) [340].

Grape-specific serum IgE level was 2.29 KUA/l (Caiaffa et al. 2003) [708].

Grape-specific serum IgE levels were between 0.95 and 18.9 KUA/l (Pastorello et al. 2003) [707]

SDS-PAGE was performed on a 10% polyacrylmide gel (Senna et al. 2001) [706]

The extracts were separated in a discontinuous buffer system in an SDS-polyacrylamide gradient gel with a 6% stacking gel and a 7.5% to 20% separation gradient gel under reducing conditions (Pastorello et al. 2003) [707]

Proteins were transferred onto a nitrocellulose membrane. The membrane was saturated with 5% deffated dried milk before incubation with patients’ sera (1:2). Bound IgE was detected by peroxidase-conjugated anti-human IgE serum (Senna et al. 2001) [706]

The separated proteins were electroblotted onto a nitrocellulose membrane. The nitrocellulose was incubated with each patient's serum diluted 1:4 in blocking solution. The bound specific antibodies were detected by means of incubation with iodine 125–labeled anti-human IgE antiserum and exposure on x-ray film (Pastorello et al. 2003) [707]

A 31 kDa protein was recognised by IgE from the sera of the 3 patients (Anton et al. 1997) [961].

A 94 kDa protein was recognised by IgE in sera from a single patient (Rodriguez et al. 2001) [705].

A 30 kDa grape protein was recognised by IgE in sera from a single patient (Senna et al. 2001) [706]

The patient had IgE against a 13 kDa protein (Garcia-Robaina et al. 2001) [340].

IgE binding at proteins of 30 kDa (11/14, 79%), 9 kDa (10/14, 71%), and 24 kDa (3/14, 21%). Latex IgE immunoblotting shows that only 6 patients had positive IgE binding, whereas the others did not (Pastorello et al. 2003) [707]

A 94 kDa grape protein was recognised by serum IgE from patient no. 8. Patient no. 9 bound to 100, 60, 34, 28, 24 and 17 kDa proteins bands, patient no. 12 to 31 and 24 kDa, no. 13 to a 34 kDa and no. 14 to a 17 kDa (Rodriguez et al. 2004) [960].

A piece of grape pulp applied in the internal face of the lower lip for 10 min (Rodriguez et al. 2001) [705].

Muscat de Hamburg’ grapes (Rodriguez et al. 2004) [960].

Lip open challenge (Rodiguez et al. 2001) [705].

Open (Rodriguez et al. 2004) [960].

A 5-year-old female (Rodriguez et al. 2001) [705].

Two children (Rodriguez et al. 2004) [960].

Erythema and vesicles in the contact area (Rodriguez et al. 2001) [705].

Positive in 2 of 2 patients (Rodriguez et al. 2004) [960].