Oropharyngeal sympotoms characteristic of the oral allergy syndrome (OAS) (Pastorello et al. 1994) [156], (Pastorello et al. 2001) [152] and systematic symptoms (Pastorello et al. 2000) [150]
In addition, Rodriguez et al. (2000) [491] described generalised anaphylaxis
Skin Prick Test
Number of Studies:
1-5
Food/Type of allergen:
Fresh fruit and commercial apricot extracts (Pastorello et al. 1994) [156]
Fresh fruit (Rodriguez et al. 2000) [491] (Pastorello et al. 2000) [150]
Protocol:
(controls, definition of positive etc)
Pastorello et al. (1994) [156] and Pastorello et al. (2000) [150] used histamine dihydrochloride (10 mg/mL) as a positive control, and the glycerol-containing diluent of the prick solution as a negative control. A wheal graded at least 2+was regarded as positive.
Skin prick tests (SPTs) were carried out by the prick-prick technique. Histamine (10 mg/mL) and saline solution were used as positive and negative controls repectively. A positive SPT result was defined as a mean (average of orthogonal to largest diameter) wheal of 3 mm or greater (after subtracting the diameter of the wheal induced by the diluent control). (Rodriguez et al. 2000) [491]
18 of 28 patients had specific IgE to apricot (Rodriguez et al. 2000) [491]
All patients had specific IgE to apricot (Pastorello et al. 2000) [150]
Immunoblotting
Immunoblotting separation:
The extracts were separated in a discontinuous buffer system in an SDS-polyacrylamide gradient gel with a 6% stacking gel and a 7.5% to 20% separation gradient. Samples were boiled and reduced with beta-mercaptoethanol (Pastorello et al. 1994) [156], (Pastorello et al. 2000) [150]
Immunoblotting detection method:
The proteins were electroblotted to a nitrocellulose membrane, pore size 0.2 to 0.45 µm using a Trans-Blot Cell. The membrane was blocked with phosphate-buffered saline pH 7.4 with 0.5% (v/v) Tween 20 and incubated with the sera (diluted 1:4). The IgE-binding components were detected with iodine 125–labeled anti-human IgE antiserum diluted 1:4 (Pastorello et el. 1994) [156], (Pastorello et al. 2000) [150]
Immunoblotting results:
87.5% of sera (14 of 16) of Pastorello et al. 1994 [156], showed Ig E binding to a 13 kDa protein and 77% (14 of 18) to a 30 kDa protein . The other IgE -binding components were: 70 kDa (6 of 16, 37.5%), 20 kDa (5 of 16, 31%), 14 kDa (4 of 16, 25%), 40 kDa (4 of 16, 25%) and 30 kDa (2 of 16, 12.5%). Only the component at 13 kDa was detectable in patients with negative responses to birch pollen.
IgE immunoblotting in sera from the 30 patients showed IgE binding to proteins with molecular weights of 9 kDa (30 patients, 100%), 15 kDa (4 patients, 13.3%), 19 kDa (12 patients, 40%), 30 kDa (3 patients, 10%), 43 kDa (6 patients, 20%), 67 kDa (7 patients, 23.3%), and 80 kDa (4 patients, 13.3%) (Pastorello et al. 2000) [150]
Oral provocation
Number of Studies:
1-5
Food used and oral provocation
vehicle
Apricot fruit (Pastorello et al. 1994) [156], (Pastorello et al. 2000) [150]
A total of 17 g of dehydrated whole fruit was masked in a mix of orange (200 mL) and pineapple (200 mL) juices, sugar (16 g), wheat meal (13 g), and liquid coloring (Rodriguez et al. 2000) [491]
Blind?
No. (Pastorello et al. 1994) [156], (Pastorello et al. 2000) [150]
Patients chewed the fruit for 1 minute and then spat it out. If no symptoms appeared within 15 minutes, the challenge was repeated with increasing amounts from 4 mg up to 64 g. Patients were asked not to spit out the last two doses but to swallow the fruit instead. (Pastorello et al. 1994) [156], (Pastorello et al. 2000) [150]
Symptoms
Oropharyngeal sympotoms (itching or tingling of the lips or oral mucosa) in 6 patients (Pastorello et al. 1994) [156]
3 patient had oropharyngeal sympotoms and two generalised anaphylaxis (Rodriguez et al. 2000) [491]
All patients reported OAS, and 2 reported systemic symptoms to apricot (Pastorello et al. 2000) [150]
IgE cross-reactivity and Polysensitisation
Clinically relevant cross-reactivity between apricot and peach has been observed by immunoblot inhibition (Pastorello et al. 1994) [156].
Other Clinical information
Reviews (0)
References (4)
Pastorello EA, D'Ambrosio FP, Pravettoni V, Farioli L, Giuffrida G, Monza M, Ansaloni R, Fortunato D, Scibola E, Rivolta F, Incorvaia C, Bengtsson A, Conti A, Ortolani C
Evidence for a lipid transfer protein as the major allergen of apricot. J Allergy Clin Immunol 105:371-377 2000
PUBMED ID:
10669861
Pastorello EA, Farioli L, Pravettoni V, Giuffrida MG, Ortolani C, Fortunato D, Trambaioli C, Scibola E, Calamari AM, Robino AM, Conti A.
Characterization of the major allergen of plum as a lipid transfer protein. J Chromatogr B Biomed Sci Appl 756(1-2):95-103 2001
PUBMED ID:
11419731
Pastorello EA, Ortolani C, Farioli L, Pravettoni V, Ispano M, Borga A, Bengtsson A, Incorvaia C, Berti C, Zanussi C.
Allergenic cross-reactivity among peach, apricot, plum, and cherry in patients with oral allergy syndrome: an in vivo and in vitro study. J Allergy Clin Immunol 94(4):699-707 1994
PUBMED ID:
7930303
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