Food:

CLINICAL INFORMATION for Pomegranate

Clinical History
Number of Studies:
1-5
Number of Patients:
1-5
Symptoms:
Symptoms include oral allergy syndrome, abdominal pain, generalised or facial urticaria, anaphylactic shock, laryngeal oedema, shortness of breath, bronchospasm, tongue angioedema (Igea et al. 1991 [825]; Gaig et al. 1992 [336]; Gaig et al. 1999 [58]).

Skin Prick Test
Number of Studies:
1-5
Food/Type of allergen:

Fruit (Gaig et al. 1999 [58]; Igea et al. 1991 [825]).

Fresh fruit and liquefied fruit (Gaig et al. 1992) [336]

Protocol:
(controls, definition of positive etc)

Use of the prick to prick technique (Igea et al. 1991 [825]; Gaig et al. 1992 [336]; Gaig et al. 1999 [58]).

1% histamine was used as positive control (Gaig et al. 1992) [336].

Number of Patients:

Two 25-year-old women and a 3-year-old child (Gaig et al. 1999) [58].

A 7-year-old girl (Gaig et al. 1992) [336]

A 85-year-old woman (Igea et al. 1991) [825]
Summary of Results:

SPT was positive in the three patients (Gaig et al. 1999) [58].

SPT was positive (Gaig et al. 1992) [336].

SPT was negative after 15 min, 6 and 24 h (Igea et al. 1991) [825]

IgE assay (by RAST, CAP etc)
Number of Studies:
1-5
Food/Type of allergen:

Pomegranate pulp extract (Gaig et al. 1999) [58].

Pomegranate extract (juice) (Igea et al. 1991) [825]
IgE protocol:
RAST and immunoblotting (Gaig et al. 1999) [58].

RAST; Positivity was accepted when it was class 2 (0.7-3.5 PRU/ml) (Gaig et al. 1992) [336].

EIA (positivity was accepted between 4.8 and 160 IU/ml), RAST and histamine release test (Igea et al. 1991) [825]
Number of Patients:

Two 25-year-old women and a 3-year-old child (Gaig et al. 1999) [58].

A 7-year-old girl (Gaig et al. 1992) [336]

A 85-year-old woman (Igea et al. 1991) [825]
Summary of Results:

RAST assays were positive in 2/3 patients (3.21 and 0.75 PRU/ml) (Gaig et al. 1999) [58].

RAST was positive (0.8 PRU/ml) (Gaig et al. 1992) [336]

EIA was positive but RAST and histamine release assays were negative (Igea et al. 1991) [825]

Immunoblotting
Immunoblotting separation:
Protein separation was performed using 12.5% acrylamide 1D SDS-PAGE gels. Samples were reduced with beta-mercaptoethanol (Gaig et al. 1999) [58].
Immunoblotting detection method:
Proteins were transferred to PVDF membranes in buffer. Membranes were blocked , incubated with sera (undiluted) and bound IgE detected by peroxidase conjugated rabbit antihuman IgE. Blots were developed by chemiluminescence (Gaig et al. 1999) [58].
Immunoblotting results:

Each of the 3 patients reacted only with a 29 kDa protein (Gaig et al. 1999) [58].

Oral provocation
Number of Studies:
1-5
Food used and oral provocation vehicle
Pomegranate seeds were crushed and mixed with 100 cl of strawberry yoghourt (Igea et al. 1991) [825]
Blind?
Yes
Number of Patients?

A 85-year-old woman (Igea et al. 1991) [825]
Dose response
A single dose was given daily and the dose was increased 2-fold in the subsequent days. The challenge was positive with 40 g of pomegranate (Igea et al. 1991) [825]
Symptoms
Tongue angioedema developed 4 h after (Igea et al. 1991) [825]

IgE cross-reactivity and Polysensitisation

Other Clinical information

Reviews (0)

References (4)

Gaig P, Bartolome B, Lleonart R, Garcia-Ortega P, Palacios, R, Richart C
Allergy to pomegranate (Punica granatum) Allergy 54:287-288. 1999
PUBMED ID: 10321568		 [58]
Gaig P, Botey J, Gutierrez V, pena M, Eseverri JL, Marin A
Allergy to pomegranate (Punica granatum) J Investig Allergol Clin Immunol 2:216-218. 1992
PUBMED ID: 1342903		 [336]
Garcia J, Carreno A, Contreras L et al
Alergia a la granda. Revision de nuestra casuistica Rev Esp Alergol Immunol Clin 11:Suppl 2 146. 1996
PUBMED ID: unknown		 [338]
Igea JM, Cuesta J, Cuevas M, Elias LM, Marcos C, Lazaro M, Compaired JA
Adverse reaction to pomegranate ingestion Allergy. 46(6):472-4. 1991
PUBMED ID: 1957996		 [825]
This record was last modified on 18-Oct-2006
Page processed in 0.047 seconds
To top of page
Home Database Search Informall project Contact us