Fritsch et al. (1997) [56] report that positive SPTs with pumpkin seed were found with 2/3 patients. All 3 patients were SPT positive for a wide variety of allergens.
Figueredo et al. (2000) [54] report positive SPTs with fresh fruits, pumpkin and other Cucurbitaceae fruits: cucumber, zucchini, muskmelon, and watermelon.
Fritsch et al. (1997) [56] report RAST class 2 for pumpkinseed with sera from each of the 3 patients. One of the sera was also class 2 for grass pollen, one was class 3 grass pollen and birch pollen while one was class 5 for grass pollen, class 3 for birch, class 3 for mugwort and class 2 for wasp and bee venoms.
Immunoblotting
Immunoblotting separation:
Fritsch et al. (1997) [56] used 5% stacking and 12% separating SDS-PAGE gels.
Figueredo et al. (2000) [54] used 15% and 5% SDS-PAGE for separating and stacking gels.
Immunoblotting detection method:
Fritsch et al. (1997) [56] transfered proteins to nitrocellose and used rabbit anti-IgE for detection.
Immunoblotting results:
Fritsch et al. (1997) [56] report pumpkinseed allergens of 13, 14, 36, 48, 77, and 87 kDa. Inhibition experiments with recombinant birch profilin were performed: IgE binding to the 14-kDa allergen was completely blocked by preincubation of the sera with recombinant birch profilin.
Figueredo et al. (2000) [54] reported eight IgE binding bands in the pumpkin extracts in the range of 8 to 109 kDa. Immunoblotting to other Cucurbitaceae extracts showed several IgE-binding bands common with pumpkin: muskmelon (38.9 and 79.4 kDa), watermelon (8.9, 13.8, and 67.6 kDa), cucumber (8.9 and 13.8 kDa), and zucchini (8.9 kDa).
Oral provocation
Number of Studies:
0
Food used and oral provocation
vehicle
N/A
Blind?
N/A
Number of Patients?
N/A
Dose response
N/A
Symptoms
No oral provocation performed
IgE cross-reactivity and Polysensitisation
Fritsch et al. (1997) [56] report inhibition of IgE binding to the 14 kDa allergen by birch profilin, Bet v 2, showing that some anti-pumpkin IgE binds to Bet v 2. It is not clear if allergy to birch pollen might lead to pumpkin allergy as the binding affinities may not be high enough to cause clinically relevent cross-reactivity.
Other Clinical information
Fritsch et al. (1997) [56] report allergy to roasted pumpkinseed, with sensitisation probably by inhalation of pumpkinseed flour used as fishing bait. No allergy to pumpkin flesh was observed.
Figueredo et al. (2000) [54] report allergy to the cooked fibrous part of pumpkin.
Reviews (0)
References (2)
Figueredo E, Cuesta-Herranz J, Minguez A, Vidarte L, Pastor C, de las Heras M, Vivanco F, Lahoz C
Allergy to pumpkin and cross-reactivity to other Cucurbitaceae fruits. J Allergy Clin Immunol 106: 402-403. 2000
PUBMED ID:
10932088
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