Petrus et al. (1999) [1751] report a case of a 5 year old boy who suffered Quincke oedema after eating squid.
Carrillo et al. (1992) [1556] reported seven patients who reacted after ingesting squid or inhaling vapors from cooking squid. Castillo et al. (1994) [1759] extended this series to 24 and Castillo et al. (1996) [1758] to 33 patients. Symptoms included urticaria/angioedema, asthma, rhinitis, gasterointestinal and anaphylactic reactions.
Miyake et al. (1987) [1756] and Miyake et al. (1988) [1757] report a case of exercise-induced anaphylaxis after eating both shrimp and squid. The 13 year old boy was sensitized to shirmp, squid, crab, octopus, clam and short-neck clam.
Skin Prick Test
Number of Studies:
1-5
Food/Type of allergen:
Carrillo et al. (1992) [1556], Castillo et al. (1994) [1759] and Castillo et al. (1996) [1758] used PBS extracts of raw and cooked squid, octopus and limpet which were centrifuged and passed through 0.22 µm pore filters (Millipore) together with commercial extracts of aeroallergens (cockroach), shrimp, lobster, crab, mussel and clam.
Protocol:
(controls, definition of positive etc)
Carrillo et al. (1992) [1556], Castillo et al. (1994) [1759] and Castillo et al. (1996) [1758] attributed a positive raction when wheals ≥3 mm developed after 15-20 minutes.
Number of Patients:
Carrillo et al. (1992) [1556] tested 7 patients, Castillo et al. (1994) [1759] tested 35 shellfish allergic patients and Castillo et al. (1996) [1758] tested 107 patients.
Castillo et al. (1994) [1759] reported that there was no significant difference between raw and boiled squid or octapus. 73.7% of squid positive patients also reacted to cockroach. There was a strong association of SPT positives between shrimp, squid, lobster and crab (18 patients). There was also a significant association for positive reactions to squid, octapus, mussel and clam.
Carrillo et al. (1992) [1556] reported that all 7 patients gave strongly positive SPTs to squid and crustaceans (shrimp, lobster and crab).
Miyake et al. (1988) [1757] found positive SPTs with shrimp, squid, crab, octopus, clam and short-neck clam.
IgE assay (by RAST, CAP etc)
Number of Studies:
1-5
Food/Type of allergen:
Miyazawa et al. (1996) [1558] used 100 g fresh squid, cut into small pieces with scissors and boiled in distilled water (300 ml) at 100° C for 10 minutes, then centrifuged at 20,000 x g for 20 minutes.
Carrillo et al. (1992) [1556], Castillo et al. (1994) [1759] and Castillo et al. (1996) [1758] used PBS extracts of raw and cooked squid which were centrifuged and passed through 0.22 µm pore filters (Millipore).
IgE protocol:
Miyazawa et al. (1996) [1558] used CAP and immunoblotting.
Carrillo et al. (1992) [1556], Castillo et al. (1994) [1759] and Castillo et al. (1996) [1758] used CAP or RAST.
Number of Patients:
Miyazawa et al. (1996) [1558] used sera from 4 squid allergic patients and 7 shrimp allergic patients.
Summary of Results:
Petrus et al. (1999) [1751] reported RAST class 4 for dust mite, class 3 for squid, shrimp and Cocksfoot grass pollen.
Carrillo et al. (1992) [1556] report that all 7 patients were RAST positive for squid and for crustacea. IgE cross-reactivity between squid and shrimp and other crustaceans was demonstrated by reverse immunoassay inhibition studies. Cross-reactivity could not be demonstrated between squid and octopus nor between squid and other mollusks.
Castillo et al. (1994) [1759] report a poor correlation between SPT and RAST results for squid, shrimp and clam.
Immunoblotting
Immunoblotting separation:
Miyazawa et al. (1996) [1558] separated proteins using 1-D 5% to 15% gradient SDS-PAGE gels under reducing conditions.
Immunoblotting detection method:
Miyazawa et al. (1996) [1558] transferred the proteins to a polyvinylidene difluoride membrane (Bio-Rad Laboratories). The membrane was rinsed in PBS, and blocked with 1% (w/v) bovine serum albumin (BSA) in PBS. After another rinse with PBS containing 0.02% (v/v) Tween-20, it was cut into strips, which were incubated overnight with the patients’ pooled serum diluted 1:2 (v/v). After being washed, the strip was incubated with anti-human IgE antibody–conjugated alkaline phosphatase (Quidel, San Diego, Calif.) overnight, and stained with 5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium.
Immunoblotting results:
Miyazawa et al. (1996) [1558] reported that the abundant 38 kDa protein from extracts of boiled squid bound IgE from squid allergic patients. There was cross-reactivity between the squid 38 kDa protein and shrimp tropomyosin using IgE or a murine monoclonal antibody.
Oral provocation
Number of Studies:
1-5
Food used and oral provocation
vehicle
No reported.
Blind?
Petrus et al. (1999) [1751] used open labial challenge.
Number of Patients?
Petrus et al. (1999) [1751] challenged 1 child.
Dose response
No reported.
Symptoms
Petrus et al. (1999) [1751] report a strongly positive labial challenge with labial oedema, swelling and intense itching.
IgE cross-reactivity and Polysensitisation
Castillo et al. (1994) [1759] suggest that there was IgE cross-reactivity between squid and cockroach; shrimp, squid, lobster and crab (also shown by Miyazawa et al. 1996 [1558]); and squid, octapus, mussel and clam. Castillo et al. (1996) [1758] suggest that the squid - cockroach link might be due to a common allergen with house dust mites. Petrus et al. (1999) [1751] also shows a link to house dust mites.
Other Clinical information
The Pharmacia squid allergen f58 is from Todarodes pacificus and f258 is from Loligo spp.
Tabka et al. (1998) [1750] and Goday Bujan et al. (1991) [1760] report cases of occupational allergy to squid. Tabka et al. (1998) [1750] cases included an individual with food allergy to shrimp.
Reviews (0)
References (11)
Carrillo T, Castillo R, Caminero J, Cuevas M, Rodriguez JC, Acosta O, Rodriguez de Castro F.
Squid hypersensitivity: a clinical and immunologic study. Ann Allergy. 68(6):483-487.
1992
PUBMED ID:
1610023
Jeoung BJ, Reese G, Hauck P, Oliver JB, Daul CB, Lehrer SB.
Quantification of the major brown shrimp allergen Pen a 1 (tropomyosin) by a monoclonal antibody-based sandwich ELISA. J Allergy Clin Immunol. 100(2):229-234. 1997
PUBMED ID:
9275145
Miyake T, Kawamori J, Yoshida T.
[Detection of specific IgE antibodies to squid by radioimmunoassay with squid extract for scratch testing: a case of anaphylactic shock induced by squid.] Jpn J Pediatr Allergy Clin Immunol. 1:14-17 1987
PUBMED ID:
unknown
[1756]
Miyazawa H, Fukamachi H, Inagaki Y, Reese G, Daul CB, Lehrer SB, Inouye S, Sakaguchi M.
Identification of the first major allergen of a squid (Todarodes pacificus). J Allergy Clin Immunol. 98(5 Pt 1):948-953.
1996
PUBMED ID:
8939158
Petrus M, Nyunga M, Causse E, Chung E, Cossarizza G.
Allergie au calmar et aux acariens chez l'enfant Arch Pediatr. 6(10):1075-1076.
1999
PUBMED ID:
10544783
Tabka F, Choudat D, Vacher JG, Thomas-Alliel S, Martin JC, Conso F
Allergie immediate au calmar Deux observations REVUE FRANCAISE D ALLERGOLOGIE ET D IMMUNOLOGIE CLINIQUE 38 (8): 713-715
1998
PUBMED ID:
unknown
[1751]
This record was last modified on 18-Oct-2006
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