Willerroider et al. (2003) [914] reported the clinical symptoms of thirty four patients with a history of allergy to bell pepper as follows. Rhinoconjunctivitis (50%), OAS (38%), urticaria (32%), allergic asthma (24%), diarrhea (6%), angio-oedema (3%), cephalea (6%) and exacerbation of atopic eczema dermatitis (3%).
Jensen-Jarolim et al. (1998) [913] reported the clinical symptoms of 11 patients as follows: Irritation of the oral mucosa (2/11), pruritus, urticaria, angioedema, atopic dermatitis (9/11), nausea, vomiting, diarrhea (6/11), shortness of breath (3/11).
Skin Prick Test
Number of Studies:
1-5
Food/Type of allergen:
Eight different types of bell peppers and paprika powder extracts (Jensen-Jarolim et al. 1998) [913]
Protocol:
(controls, definition of positive etc)
Not specified
Number of Patients:
11 patients suffering from food adverse reactions to bell pepper and paprika (Jensen-Jarolim et al. 1998) [913]
Summary of Results:
3/11 patients showed positive reactivity in skin prick testing with paprika extract (Jensen-Jarolim et al. 1998) [913]
IgE assay (by RAST, CAP etc)
Number of Studies:
1-5
Food/Type of allergen:
Paprika powder extracts (Jensen-Jarolim et al. 1998) [913]
IgE protocol:
RAST
Number of Patients:
Eleven patients suffering from food adverse reactions to bell pepper and paprika (Jensen-Jarolim et al. 1998) [913]
Summary of Results:
8/11 patients were positive in paprika RAST (Jensen-Jarolim et al. 1998) [913]
Immunoblotting
Immunoblotting separation:
Bell pepper protein extract was separated on either 12% or 7.5-20% polyacrylamide gels under reducing conditions (Willerroider et al. 2003) [914]
Bell pepper protein extract was separated on 12% homogeneous gel and 5% stacking gels (Leitner et al. 1998) [916]
Bell pepper protein extract was separated on 12% homogeneous gel and 5% stacking gels under reducing conditions (Jensen-Jarolim et al. 1998) [913]
Immunoblotting detection method:
The proteins were transferred onto nitrocellulose membranes. The membrane was incubated with patient sera (1:4). Bound IgE was detected by 125I-labelled anti-human IgE antibodies(1:20) and visualised by autoradiography (Willerroider et al. 2003) [914]
The proteins were transferred onto nitrocellulose membranes. The membrane was blocked with 0.5% bovine serum albumin and incubated with patient sera (1:4). Bound IgE was detected by 125I-labelled rabbit anti-human IgE antibodies (Leitner et al. 1998) [916]
The proteins were transferred onto nitrocellulose membranes. The membrane was blocked with 0.5% bovine serum albumin and incubated with patient sera (1:4). Bound IgE was detected by 125I-labelled rabbit anti-human IgE antibodies (Jensen-Jarolim et al. 1998) [913]
Immunoblotting results:
All the patients displayed IgE binding to a range of bell pepper proteins from 14 to 90 kDa. 32% reacted to the 14 kDa protein (profilin) (Willerroider et al. 2003) [914]
73 % (16/22) sera of patients with celery-birch-mugwort-spice syndrome demonstrated IgE binding to pepper extract. 93% of those patientes recognised proeins at 28 and 60 kDa. Two sera showed additional IgE binding to minor allergens of 14, 25, 30, 35 and 40 kDa. One serum reacted only to the 60 kDa allergen. 95 % sera of patients with celery-birch-mugwort-spice syndrome demonstrated IgE binding to paprika allergens in the range of 23 to 50 kDa (Leitner et al. 1998) [916]
IgE binding patterns varied among the eight different types of bell pepper extract used. Immunoblotting revealed IgE binding to 10, 14, 17, 23, 24, 28, 31, 35, 40, 46 and 69 kDa proteins. 5 of 10 patients showed intensive IgE binding to a 14 kDa protein, 10 of 10 to the 23 kDa. One patient showed only IgE binding to a 17b kDa protein. (Jensen-Jarolim et al. 1998) [913]
Oral provocation
Number of Studies:
0
Food used and oral provocation
vehicle
Blind?
Number of Patients?
Dose response
Symptoms
No oral provocation performed
IgE cross-reactivity and Polysensitisation
Ebner et al. (1998) [915] found immunologic cross-reactivity between allergens in spices from the botanic families Apiaceae, Piperaceae, and Solanaceae.
Van Toorenenbergen et al. (2000) detected profilin specific IgE only in food-allergic patients but not in pollen- allergic patients.
Other Clinical information
Reviews (0)
References (5)
Ebner C, Jensen-Jarolim E, Leitner A, Breiteneder H
Characterization of allergens in plant-derived spices: Apiaceae spices, pepper (Piperaceae), and paprika (bell peppers, Solanaceae). Allergy. 53(46 Suppl):52-4 1998
PUBMED ID:
9825999
Jensen-Jarolim E, Santner B, Leitner A, Grimm R, Scheiner O, Ebner C, Breiteneder H
Bell peppers (Capsicum annuum) express allergens (profilin, pathogenesis-related protein P23 and Bet v 1) depending on the horticultural strain Int Arch Allergy Immunol. 116(2):103-9 1998
PUBMED ID:
9652302
Leitner A, Jensen-Jarolim E, Grimm R, Wuthrich B, Ebner H, Scheiner O, Kraft D, Ebner C.
Allergens in pepper and paprika. Immunologic investigation of the celery-birch-mugwort-spice syndrome Allergy. 53(1):36-41 1998
PUBMED ID:
9491227
van Toorenenbergen AW, Waanders J, Gerth Van Wijk R, Vermeulen AM
Immunoblot analysis of IgE-binding antigens in paprika and tomato pollen. Int Arch Allergy Immunol. 122(4):246-50 2000
PUBMED ID:
10971114
Willerroider M, Fuchs H, Ballmer-Weber BK, Focke M, Susani M, Thalhamer J, Ferreira F, Wuthrich B, Scheiner O, Breiteneder H, Hoffmann-Sommergruber K
Cloning and molecular and immunological characterisation of two new food allergens, Cap a 2 and Lyc e 1, profilins from bell pepper (Capsicum annuum) and Tomato (Lycopersicon esculentum). Int Arch Allergy Immunol. 131(4):245-55 2003
PUBMED ID:
12915767
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