Karlsson et al. (2004) [1133] reported the symptoms of 8 patients as follows: rhinitis (7/8), asthma (6/8) and eczema (5/8). One patient also reported stomach pain, flatulence and diarrhoea.

Rodriguez et al. (2000) [491] reported a single patient with severe anaphylaxis after ingestion of strawberry.

Wadee et al. (1990) [1142] reported a single patient with rhinitis and pharyngeal itching after eating strawberries (or several other fruits). The most severe symptoms actually occurred with cling peaches.

Karlsson et al. (2004) [1133] used commercial extracts.

Rodriguez et al. (2000) [491] used commercial almond extracts and fresh fruits (apricot, plum, strawberry, apple, peach, and pear).

Karlsson et al. (2004) [1133] used histamine as the positive control.

Rodriguez et al. (2000) [491] defined a positive skin prick test result if the mean (average of orthogonal to largest diameter) wheal was 3 mm or greater (after subtracting the diameter of the wheal induced by the diluent control). Negative and positive controls for skin testing were saline solution and histamine dihydrochloride (10 mg/mL), respectively. Tests with fresh fruits were performed by the prick-prick technique.

Karlsson et al. (2004) [1133] report SPT results with strawberry for a single patient.

Rodriguez et al. (2000) [491] tested 31 patients with strawberry extract.

Karlsson et al. (2004) [1133] reported comparative scoring for strawberry, histamine control and birch was +++(+), +++ and ++, respectively.

Rodriguez et al. (2000) [491] reported that 13/31 patients had a positive SPT to strawberry (this was fewer than for peach, plum, pear, apple, almond or apricot).

Frozen strawberries were thawed for 10 min. and homogenized. Cellular debris was removed by centrifugation. The concentration of protein was approximately 0.5 mg/ml extract.
A protein enriched extract was made from strawberries frozen in liquid nitrogen and ground into a fine powder using mortar and pestle. 200 mg was extracted for 15 min at 4°C with 500 µl Tris/HCl pH 6.8, 0.7 M sucrose, 50 mM EDTA, 0.1 M KCl, 0.33g polypyrolidone and protease inhibitor cocktail (Complete ^TMMini, Roche). 500 µl phenol was added and the sample was thoroughly mixed by vortexing. After centrifugation at 15000 x g for 5 min., the phenol phase (upper phase) was collected. The proteins in the upper phase were precipitated by adding 5 vol of 0.1M ammonium acetate in methanol at -20°C. The precipitate was washed by repeated resuspension in 0.1M ammonium acetate in methanol at -20°C (X 2) and and acetone at -20°C (X 2). The protein precipitate was resolved in buffer and centrifuged at 15000 x g for 20 min. The yield of protein was approximately 200 µg per 200 mg frozen strawberry powder.
Birch pollen, apple and timothy grass pollen were commercial extracts (Karlsson et al. 2004) [1133].

Rodriguez et al. (2000) [491] used commercial extracts.

Karlsson et al. (2004) [1133] tested sera from 8 patients.

Rodriguez et al. (2000) [491] tested sera from 34 patients.

Karlsson et al. (2004) [1133] report that 4 patients were class 0, 1 patient class 1 and 3 patients were class 2 for specific IgE to strawberry. 1 patient was class 0, 1 patient was class 2 and 6 were class 4 for specific IgE to birch pollen. 2 patient were class 0, 1 patient was class 1 and 4 were class 2 and 1 was class 3 for specific IgE to apple. 4 patients were class 0, and 4 patients were class 3 for specific IgE to timothy grass pollen.

Rodriguez et al. (2000) [491] report that sera from 14/34 patients were positive by CAP System FEIA.

Karlsson et al. (2004) [1133] separated strawberry extract (approximately 150 µg protein) by SDS-PAGE (NuPAGE 4-12% BisTris, Invitrogen).

Wadee et al. (1990) [1142] used a 5-20% acrylamide gradient separating gel with a 4% stacking gel.

Karlsson et al. (2004) [1133] transferred proteins to a polyvinylidene difluoride (PVDF) membrane and detected IgE binding with the Western Breeze Chromogenic Western Blot Immunodetection kit (Invitrogen), using as primary antibody 50 µl of patient's serum diluted to 500 µl, and as secondary antibody 500 µl of a reagent containing alkaline phosphatase-labelled murine monoclonal anti-human IgE antibody.

Wadee et al. (1990) [1142] electrophoretically transferred proteins onto a nitrocellulose sheet in a BioRad transblotting cell (60V for 3h). The membranes were quenched with 3% w/v gelatine in 2 mM Tris and 100 mM glycine and washed in Tris buffered saline with Tween 20. Strips were then incubated with 1:100 v/v dilution of patient's sera for 2 h. Membranes were washed twice in Tris buffered saline with Tween 20 and incubated with a 1:1000 v/v dilution of goat antihuman IgE horseradish peroxidase conjugate. Colour was developed with the horseradish peroxidase substrate (Bio-Rad).

Karlsson et al. (2004) [1133] report that sera from 5/8 patients showed IgE binding to a double band at 18 and 20 kDa, which were identified by mass spectroscopy as strawberry Bet v 1 homologues. One sera also identified a 50 kDa IgE binding band as well as the doublet using the protein enriched extract. Binding to strawberry proteins could be completely inhibited by addition of recombinant Bet v 1 or apple Mal d 1 but not by an extract timothy pollen or by BSA.

Wadee et al. (1990) [1142] report that IgE from the patient's sera bound a 30 kDa protein from strawberry, banana, guava, mandarin and peach.

Yes after initial open challenge.